Site: The General Hospital Corporation Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham. Job Summary We are seeking an experienced Research Technologist to lead our noninvasive fetal sequencing project (NIFS). Standard noninvasive prenatal testing (NIPT) relies on cell-free DNA (cfDNA) analysis from maternal plasma but is restricted to detecting common aneuploidies and some microdeletions. We have developed a high-resolution, noninvasive fetal sequencing approach that can detect clinically significant fetal variants with comparable accuracy to NIPT, eliminating the procedural risk of invasive testing. The candidate will be responsible for optimizing our current NIFS protocol to improve our cfDNA recovery and to validate those results against the current diagnostic gold standard. The Talkowski Laboratory in the Center for Genomic Medicine at Massachusetts General Hospital (MGH) and the Stanley Center for Psychiatric Research at the Broad Institute of MIT and Harvard are seeking a motivated, enthusiastic, experienced leader to join our research teams. Our group explores the impact of genomic variation on human disease, with a particular focus on the relationship between genome structure and function. We are dedicated to the development and application of innovations in genome sequencing technologies and genetic diagnostics. Our research has shown that we can use NGS techniques to more fully capture prenatal. The candidate will be expected to have experience or a high capacity to learn the following techniques: • Handling of human blood-derived samples, including isolation of plasma and buffy coat under sterile and reproducible conditions. • Sample processing, labeling, and biobanking under rigorous quality control and contamination-prevention measures consistent with good laboratory practice (GLP) standards. • Extraction and robust handling of cell-free DNA (cfDNA) and genomic DNA (gDNA) from plasma, blood, and human tissues. • Next-generation sequencing (NGS) library preparation for low-input cfDNA/gDNA samples and target hybridization protocols for exome and custom sequencing panels. • Sanger sequencing, gel electrophoresis, PCR, and qPCR-based assays for cfDNA quantification, variant detection, and workflow validation. • NGS library quality control and assessment, including quantification and fragment size analysis. • Accurate sample tracking and documentation across all stages of processing and analysis. Does this position require Patient Care? No Essential Functions - Perform DNA/RNA extraction from blood samples (incl. whole blood, buffy coat, plasma). - Perform other molecular genomics procedures as needed (i.e., sequencing library production, target hybridization, RNAseq, sample archiving, tissue culture, etc.) - Learn and perform any number of molecular genomics techniques, including single-cell RNAseq (scRNAseq), ATACseq, ChIPseq, HiC, and other methods -Independently performs non-routine, highly specialized experimental procedures. -Participates in the design and modification of research protocols. -Develops research methodologies within the parameters of experimental protocols and research objectives. -Composes and may present sections of research reports and manuscripts. -Performs advanced data analysis using advanced statistical techniques. -Responsible for the oversight and coordination of lab activities, including quality assurance. -Responsible for the oversight and coordination of the scheduling of all procedures. -Responsible for troubleshooting problems and instructing others in highly specialized techniques. Qualifications Education Bachelor's Degree Related Field of Study required Can this role accept experience in lieu of a degree? Yes Licenses and Credentials Experience Previous Research Lab Experience 3-5 years required Knowledge, Skills and Abilities - Organize and maintain a functioning research laboratory. - Troubleshoot scientific and non-scientific problems. - Proficiency in Word, PowerPoint, Excel, and Internet searches is preferred. - Well-versed in laboratory standards and practices. - May teach moderately difficult-to-complex lab techniques to students and research personnel. - May provide functional guidance to lower level technical personnel. Additional Job Details (if applicable) Remote Type Onsite Work Location 185 Cambridge Street Scheduled Weekly Hours 40 Employee Type Regular Work Shift Day (United States of America) Pay Range $62,004.80 - $90,750.40/Annual Grade 7 At Mass General Brigham, we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive, and any offer extended will take into account your skills, relevant experience if applicable, education, certifications and other essential factors. The base pay information provided offers an estimate based on the minimum job qualifications; however, it does not encompass all elements contributing to your total compensation package. In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, differentials, premiums and bonuses as applicable and recognition programs designed to celebrate your contributions and support your professional growth. We invite you to apply, and our Talent Acquisition team will provide an overview of your potential compensation and benefits package. EEO Statement: The General Hospital Corporation is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran’s Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at (857)-282-7642. Mass General Brigham Competency Framework At Mass General Brigham, our competency framework defines what effective leadership “looks like” by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused, half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline. Combat disease. Hold a hand. Help people. Impact the world. Mass General Brigham is a passionate, welcoming community where minds meet caring hearts. Come be a part of the world’s most powerful force in medicine, where every role is important in changing lives. Are you ready? Our history includes New England hospitals founded over 200 years ago, some of the first and most prestigious hospitals in the world. Built on the legacy of two leading academic medical centers, we’re more than a system—we’re leaders in the practice of medicine. Mass General Brigham is committed to serving the community. We are dedicated to enhancing patient care, teaching and research, and taking a leadership role as an integrated health care system. We recognize that increasing value and continuously improving quality are essential to maintaining excellence.